Tottenham are through to the Champions League final and could finish third in the Premier League.
Spurs must overturn a 1-0 first-leg deficit when they take on the Dutch club in the return of their last-four tie in Amsterdam, with a final against Liverpool on June 1 the prize for the winners.
Tottenham, trying to reach their first European final since the 1984 UEFA Cup, have to focus on the challenge presented by Ajax's vibrant young side.
The defender has since been given the all-clear by Spurs, who say he has not suffered a concussion
Ajax dominated the first half until Pochettino completely changed his game-plan by switching from a 3-5-2 formation to a 4-4-2 system.
The Argentine is frustrated Ajax will have an advantage ahead of Tottenham's first European semi-final since the 1984 UEFA Cup.
Guardiola's side must overturn a 1-0 first-leg deficit at the Etihad Stadium to avoid extending their manager's frustrating Champions League run.
Kane suffered a "significant lateral ligament" injury in the 1-0 first-leg victory over Manchester City on Tuesday.
Spurs face a mammoth task to rescue another season that threatens, after much promise, to end without a trophy.
A single point separates them from Manchester United, Arsenal and Chelsea in the race for the final two Champions League spots.
Pochettino pointed to Rennes, who postponed a French league game at the weekend to prepare for Thursday's Europa League tie at home to Arsenal.
After watching replays of his heated exchange, the 46-year-old Argentinian said he could offer no defence.
The Spurs boss was involved in a lengthy and heated exchange with referee Mike Dean after the loss against Burnley.
Pochettino's side had been under the cosh as Dortmund dominated the first half at Wembley, but Hugo Lloris kept them level with a series of fine saves.
Pochettino has taken to defending his failure to win silverware in almost five years at Tottenham by insisting he values qualification for Europe's elite club competition more highly.