Markram, who tested positive after first couple of practice sessions, will not be able to return to competitive arena post mandatory isolation period.
Kagiso Rabada sent back England's Chris Woakes, skipper Eoin Morgan and Chris Jordan for the tournament's third hat-trick.
South Africa now need to restrict England to 131 runs to qualify for the semifinals.
South Africa will next play Sri Lanka in their third Group 1 match on October 30, while West Indies will take on Bangladesh a day earlier.
Had it not been for Kagiso Rabada's 19, which included a one-handed six off Starc in the final over, South Africa would have got less.
One of his innings stretched into the 17th over and two into the 16th in the IPL, something he might experience in the T20 showpiece even though he bats at the top of the order.
Quinton de Kock smashed a fifty to help South Africa register an easy nine-wicket win over Sri Lanka on Sunday.
Punjab Kings named Aiden Markram as a replacement of Dawid Malan in their squad for the remainder of the Indian Premier League.
Markram has not played in a one-day international since November 2018.
Australia finished the fourth day one wicket short of victory with South Africa on 293 for nine, needing 417 to win.
Opener Dean Elgar was 128 not out at stumps, Aiden Markram made 97 on his debut, and Hashim Amla was 68 not out with Elgar at the end.