James put the Cafeteros in the lead with a spectacular effort from the edge of the penalty area in the 36th minute, thrilling a largely pro-Colombia crowd at Raymond James Stadium.
A look at Colombia's matches at this World Cup and the 2014 edition in Brazil reveals they have been more effective in total than their bigger South American opponents while also playing fewer games.
James Rodriguez remains a doubt for Colombia's World Cup opener against Japan after missing training on Friday due to muscle fatigue.
The South Americans, led by forward stars Radamel Falcao and James Rodriguez, start their Group H campaign against Japan on June 19 in Saransk.
Colombia opens Group H on Tuesday against Japan, plays the second game near its Kazan base against Poland on June 24, and concludes against Senegal on June 28.
The Boca Juniors left-back suffered a ruptured anterior cruciate ligament in his left knee during a training session in Italy.
James Rodriguez believes he has recaptured the sort of form that made him one of the stars of the 2014 World Cup.
FCF said in a brief statement that the players did work in the gym and also took part in passing and tight-space drills.