Players had reported to camp on Tuesday after being picked by new coach Montse Tomé against their will on Monday.
According to Spanish sports law, athletes are required to answer the call of their national teams unless there are circumstances that impede them from playing, such as an injury.
Prosecuting Spain football official Rubiales for World Cup kiss seems illogical, says UEFA president
Spanish state prosecutors are investigating Jenni Hermoso’s accusations against Luis Rubiales of sexual assault for the kiss at the August 20 final and coercion for then pressuring her to defend him.
Luis Rubiales arrives at Spanish court to be questioned over his kiss of player at Women's World Cup
Judge Francisco de Jorge issued the order earlier this week for Rubiales to answer his questions at Spain’s National Court.
Spanish footballer Jenni Hermoso files sexual assault complaint against Luis Rubiales over World Cup kiss
The prosecutors’ office said that, according to a new sexual consent law passed last year, Rubiales could face a fine or a prison sentence of one to four years if found guilty.
In a statement released by women players' union Futpro, Hermoso and 80 other players said they would not accept an international call-up "if the current leadership continues" at the RFEF.
In another incident on Sunday evening, broadcast around the world, Spanish football federation chief appeared to be grabbing his genitals with both hands to celebrate Spain's victory.
The Spanish football federation called an emergency meeting for Friday and activated an internal investigation into the incident, amid mounting pressure to take action against Rubiales.
Luis Rubiales kissed Jenni Hermoso on her lips after she collected her medal following the final in Sydney, provoking outrage in Spain.
Earlier Spain's sports minister on Monday had demanded that Luis Rubiales should apologise to Jenni Hermoso.