A 41-year-old woman from Kattaikonam, Thiruvananthapuram and a doctor (31) from Kumarapuram were diagnosed with the virus, Kerala health minister said in a release.
Steps including fogging and indoor spraying are being conducted across the district in a bid to control mosquito breeding.
In the wake of the rise in Zika viral infection reported in the capital, the corporation on Sunday began the one- week cleaning and mass fogging drive.
While a 35-year-old Poonthura native was detected using the newly introduced testing facility at the Medical College hospital, the 2nd case was detected in a private hospital patient.
One-year-old among those infected; 18 confirmed cases so far; testing facilities set up at 4 medical college hosps
Out of the 27 samples which were sent for testing in two batches, 26 have turned out ot be negative, health minister Veena George informed.
Measures include surveillance of Aedes mosquito and larvae, biological and chemical control
Thirteen samples out of 19 taken from different parts of the state are suspected to be Zika positive, though official confirmation is yet to arrive.