Raj, who originally hails from Tamil Nadu was criticised by a Twitter user saying that the skipper often speaks in English, Hindi and Telugu but does not use 'Tamil' to converse with people.
With this victory, India have already sealed the three-match ODI series with one match left to play.
She achieved the feat while leading India to an emphatic eight-wicket win over South Africa women in the first ODI of the three-match series.
In Smriti Mandhana's absence, debutant Priya Punia powers India to emphatic win against South Africa
It was also captain Mithali Raj's first game for India since March and with that she became the first female to last more than 20 years in international cricket, having made her debut back in 1999.
Australia's consistent performances have ensured their tight grasp on the top spots in both the women's ODI and T20I rankings tables, introduced in October last year.
Shafali, who replaced the recently retired Mithali in the T20 squad, earned her maiden call-up for the ongoing T20 series against South Africa.
The T20I series against South Africa, that begins on Tuesday in Surat, is a fresh chapter of sorts for India women.
15-year-old Shafali has been roped in to be a part of the T20I squad as what could be seen as a replacement for Mithali, who earlier announced her retirement from the shortest format of the game.
Her remarks came after she decided to retire from T20I format. Raj went on to represent India in 89 T20Is, managing to score 2,364 runs.
Tuesday was not just about Mithali Raj’s retirement in T20Is. Sooner or later, this announcement had to come.
Besides being the leading run-getter ODIs, Mithali has scored the most number of runs for India in the shortest format.
Mithali led India in 32 T20Is, including the three Women's WT20 World Cups in 2012 (Sri Lanka), 2014 (Bangladesh) and 2016 (India).
Poonam Yadav, who has taken 63 wickets in the ODI format hailed 2017 Women's World Cup as the turning point for women's cricket in the country.
The five-match series begins on September 24, while the T20 World Cup is scheduled in Australia in February-March.
Talking to TNIE in an exclusive live interview, Mithali Raj revealed that she would've been a trained Bharathanatiyam dancer if she didn't choose cricket.