Nana Patole passed the order after BJP whip Devayani Farande sought action against the person for making the video clip.
Pro-tem speaker Dilip Walse Patil made the announcement in the Assembly after which Chief Minister Uddhav Thackeray and some senior MLAs escorted Patole, 57, to the Speaker's chair.
Congress MLA Patole was elected unopposed as Maharashtra Assembly Speaker on Sunday after BJP withdrew its candidature for the post. The BJP took a decision after an all-party meeting.
Patole said that the BJP is being accorded the status of opposition party in the Assembly and Fadnavis would be the new Leader of Opposition.
The Congress announced party MLA Patole as the candidate of the ruling Shiv Sena-Congress-NCP alliance for the Speaker's post, while the BJP named Kisan Kathore as its nominee.
Maharashtra BJP chief Chandrakant Patil said Kathore, party MLA from Murbad, will be its candidate for the post.
Congress MLA Nana Patole will take on BJP's Kisan S. Kathore on Saturday ahead of the floor test for the Uddhav Thackeray-led 'Maha Vikas Aghadi' government.
Maharashtra is currently under President's rule after a stalemate over government formation following the assembly polls last month.
While a complaint about causing injury has been registered against Phuke’s supporters, Patole’s nephew has been charged with abduction and causing severe injury, police have said.
Patole, who resigned as BJP Bhandara-Gondiya MP in December, 2017, had lambasted the Narendra Modi government at the time for 'ignoring' farmers.
Fadnavis had brushed aside a question pertaining to the allegations saying that the decision was made by the state government in the year 2005, even before he got married.
Gujarat polls: Dissident BJP leader Nana Patole to join Rahul Gandhi in Ahmedabad rally, says Congress
Patole, who has been publicly attacking Prime Minister Narendra Modi, announced on Friday that he had resigned from the Lok Sabha where he represents Bhandara-Gondiya and quit the party too.
BJP Maharashtra leader Nana Patole announced on Friday that he had resigned from the Lok Sabha and quit the party too.
Patole had criticised the Centre over its policies and reportedly said Prime Minister Narendra Modi did not like to be questioned.