Ramani has accused the former minister of sexual misconduct around 20 years ago when he was a journalist while Akbar has denied the accusations.
Akbar, who appeared before Additional Chief Metropolitan Magistrate Samar Vishal, said the allegations made by Ramani were "malafide" and "defamatory".
Ramani, who appeared before Additional Chief Metropolitan Magistrate Samar Vishal, however, pleaded not guilty and claimed trial.
The court will be hearing the defamation case filed by M J Akbar, in which Priya Ramani was summoned as accused, on April 10.
Ramani was the first in a long list of women journalists to accuse the former Minister of State for External Affairs and journalist-turned-politician of sexual harassment.
Former union minister MJ Akbar filed a private criminal defamation complaint in court against Priya Ramani after his name cropped up in connection with the #MeToo Movement.
The move comes weeks after the Guild put out an updated list of its members, which included the names of Akbar, Tejpal and senior journalist Gautam Adhikari.
Akbar has been accused of sexual misconduct by several women over his tenure as a senior editor as part of the #MeToo movement, while Tejpal has been charged with rape and is currently out on bail.
The journalist gave a first-person account of the sexual harassment she faced in a November 1 article for The Washington Post.
M J Akbar appears before Delhi court to record his statement in defamation case filed against Priya Ramani
Akbar had filed the criminal defamation complaint against Ramani alleging she "wilfully" and "maliciously" made imputations against him with the ulterior motive of maligning his reputation.
Before bringing the matter to the notice of her seniors and the CM, she had warned the minister concerned but he did not care and kept on sending her messages.
The Guild also offered legal support to women journalists who have levelled the allegations against Akbar in case he does not withdraw the present case or files such cases against other women too.
Senior advocate Geeta Luthra said Akbar has resigned from the post of Minister of State for External Affairs due to these allegations and this showed the extent of damage caused to his reputation.
After Akbar filed the case on Monday, about 20 women journalists came out in support of Ramani, pledging to depose in court.
Hemmed by charges of sexual harassment from about 20 former colleagues, journalist-turned-politician M J Akbar resigned as the Minister of State for External Affairs on Wednesday.