Special judge for CBI cases VC Barde rejected the agency's plea, noting that the accused had not consented to the tests, and consent was mandatory before such tests are performed.
Nirav Modi, 48, has been lodged at Wandsworth prison in London since his arrest in March in connection with the nearly USD 2 billion PNB fraud.
635 employees from Hyderabad unit of fugitive diamantaire Mehul Chowksi owned Gitanjali gems left in the lurch
While Choksi fled the country, employees received neither pending salaries nor PF amount.
UK's Westminster Magistrate's Court last month denied bail to Modi, who has been behind bars at one of England's most overcrowded prisons, Her Majesty's Prison Wandsworth.
The 48-year-old is back in prison after his bail was denied on Wednesday by Westminster Magistrates' Court Chief Magistrate Emma Arbuthnot.
Choksi, the alleged partner-in-crime of his nephew Nirav Modi in the banking fraud, refused to say anything further on the issue, on the advice of his lawyers.
The government will hire an independent expert to reassess all the assets.
PNB scam: Court orders issuance of non-bailable warrant against absconding diamond merchant Mehul Choksi
The chargesheet names managing director of Gitanjali group Mehul Choksi, former PNB managing director and CEO Usha Ananthasubramanian and two serving executive directors of PNB.
PNB scam: CBI may soon file charge sheet against those arrested; probe against Nirav Modi, Mehul Choski still on
The probe against those absconding, such as Modi, Choksi, and their relatives, is still on, and the agency may take time to finalise their roles in the case, officials said.
However, when compared with the same period last year, the optimism index has grown by 7.6 per cent, it added.
Central Vigilance Commission awaits detailed report; calls Punjab Natioal Bank auditors in fraud case
The watchdog asserted the need for strengthening the preventive vigilance mechanism, ensuring procedures were not just routinely followed but that the veracity of reports was verified.
With more bank default cases surfacing across the country for the past few months, the bank officials are more cautious while accepting business loan applications.
CBI had Tuesday informed the court it had added 'criminal breach of trust' charge to the case related to diamond trader Mehul Choksi's firms.
PNB has taken a series of measures to check misuse of SWIFT message platform allegedly used by Nirav Modi and his associates to defraud the bank of Rs 1,2967.86 crore, the Finance Ministry said.
Punjab National Bank today said it has put in place a three-tier authentication system for SWIFT transaction and is in the process of installing a "superior" software system.