Both the high-profile openers, Kohli and Faf du Plessis, are big match players and will be itching to make an impact after low returns in the previous game.
Chasing 169 to win, the former captain looked in his element playing some of his signature wristy shots against both seamers and pacers.
Despite a fighting half-century from Rahul Tripathi (58 from 37 balls), they eventually folded out for 125 in 19.2 overs with Wanindu Hasaranga claiming five wickets for 18 runs.
In cricket, when two sides with specific weak-links are pitted against each other it makes the contest all the more exhilarating.
Kohli's form has been one of the reasons for the inconsistent show of RCB who are currently in the fifth spot with five wins from nine matches.
Thankfully for the franchise, the man who took over the reins from Kohli, Du Plessis, played a captain’s knock. His 64-ball 96 proved pivotal for RCB as they beat LSG by 18 runs.
The 37-year-old du Plessis roared back to form after a string of low scores with a 64-ball knock to help RCB post 181 for six after being sent into bat.
The former Proteas captain had 11 boundaries and two sixes in his 64-ball innings, playing shots all around the DY Patil Stadium.
Shastri said that the team's new captain Du Plessis, former skipper Virat Kohli and all-rounder Glenn Maxwell are going to play an important role this season.
Royal Challengers Bangalore skipper Faf du Plessis spoke about facing his old team and the memories he shared with the team CSK.
Faf du Plessis, who took over the reins of the side from long-serving skipper Virat Kohli, allayed fears on Tuesday that his side would likely buckle under pressure.
Maxwell, who missed the first few games due to his marriage, is expected to play against Mumbai Indians on April 9.
For RCB, skipper Faf Du Plessis is in prime form after he made a scintillating 57-ball 88 and would like to continue in the same vein.
Shahrukh Khan, who hit one four and two maximums, finished the game with a boundary as Mayank Agarwal started his captaincy tenure with a win.
RCB and PBKS will have new captains at the helm in Faf du Plessis and Mayank Agarwal respectively.
RCB will now be led by four-time IPL winner Du Plessis, who was purchased by the franchise for Rs 7 crore in the mega auction last month.
The South African also said that he found Dhoni to be complete "opposite" to what it was like captaining in South Africa.
The four-time IPL winner for CSK was on Saturday unveiled as RCB skipper to take the baton from their longest-standing captain, Virat Kohli who took charge ahead of IPL 2013.
Du Plessis was an obvious choice after Virat Kohli decided to call time on his leadership role for the franchise.
Du Plessis insisted that he wants to be a part of the history by contributing in the revival of international cricket in Pakistan.