The sanctions targeted senior security and justice officials, prominent media figures, one of Lukashenko's sons, defense-related firms and a major fertilizer exporter.
The EU had found itself unable to do much to alter the course of either nation, and therefore turned to linking money to their adherence to democratic behavior.
Poland PM Mateusz Morawiecki's appeal on Wednesday was made to a nation with a vaccination rate of just 54%.
Later on Sunday, the Polish head of government will meet his counterparts from the Baltic states -- two of which also share a border with Belarus -- to discuss the conflict.
Most are fleeing conflict or hopelessness at home and aim to reach Germany or other western European countries.
The situation marks an escalation in a tense migration and political border crisis where the lives of thousands of migrants are at stake.
EU headquarters and Poland have been in a long-running standoff over the Polish government's efforts to control the judiciary.
Police said that incident occurred Saturday evening near Kolonia Klukowicze, a village close to the border with Belarus.
Hundreds of desperate migrants are trapped in freezing temperatures on the border and the presence of troops from both sides has raised fears of a confrontation.
Poland has received strong signals of support and solidarity from the EU and EU member states and the United States as it faces its border crisis.
A spokeswoman for Poland's Border Guards, Ewelina Szczepanska, said the presence of guards, police and the military has been reinforced at the border.
The document addressed to obstetricians comes in reaction to the hospital death of a 30-year-old mother whose 22-week pregnancy had medical problems.
The protesters held portraits of the woman, 30-year-old Iza, who died in hospital in Pszczyna, southern Poland, from septic shock.
Radoslaw Fogiel, a spokesman for Poland’s nationalist ruling party, brushed the penalties off, saying Poland’s contribution into the EU coffers is much larger than that.
Morawiecki faced less sanguine criticism from his Polish political opponents, many of whom are deeply worried about Poland's increasing isolation within the EU.