It's the first time since his accident that he has been captured without a leg brace. A close-up picture of his leg shows a massive scar across his knee.
The previously announced cast members are Leonardo DiCaprio, Robert De Niro, Jesse Plemons, Lily Gladstone and Brendan Fraser.
Based on David Grann's 2017 bestseller, the film is set in 1920s Oklahoma, and centres on the Osage Nation murders, in which members of the Native American tribe were murdered one by one.
According to Variety, De Niro along with renowned producer Jane Rosenthal have launched the latest edition of the festival, which runs from June 9 to June 20.
De Niro stars alongside Leonardo DiCaprio, who plays his nephew Ernest Burkhart. The Apple film is scheduled to premiere later this year.
The details of the actor's injury are not yet known but he wasn't on the film's set when it happened.
The movie is loosely based on the life of stand-up comic Sebastian Maniscalco, according to The Hollywood Reporter.
The news comes days after Amazon Studios said it was "reviewing" its options on ‘The Romanoffs’, the eight-episode anthology from ‘Mad Men’ creator Weiner, as well as the untitled Russell drama.
Online movie streaming service Netflix has bagged the rights of Martin Scorsese's next movie starring Robert De Niro, Al Pacino, Harvey Keitel and Joe Pesci for $105 million.