Venus Williams won back-to-back U.S. Opens in 2000 and 2001, and this year marks 25 years since she reached the 1997 final in Flushing Meadows as a 17-year-old in her first full season on tour.
"I'm not looking for some ceremonial, final on-court moment," Williams wrote in Vogue. "I'm terrible at goodbyes, the world's worst."
Her most recent match on tour came at Chicago last August. She turns 42 in two weeks. Also missing from the entry list is Leylah Fernandez, the 2021 US.
Serena Williams has dominated women's tennis for more than a decade, winning 23 Grand Slams including seven titles at the All England Club.
Five-time champ Venus Williams became the latest star to crash out in a 6-2, 6-7 (5/7), 8-6 defeat against Kiki Bertens.
The seven-time Grand Slam champion has now lost both her matches at major tournaments this year, having reached the Australian Open and Wimbledon finals in 2017.
Pliskova launched her bid for a season-ending triumph at the WTA Finals with a convincing 6-2, 6-2 victory over Venus.
Romanian tennis player Simona Halep continued to lead the Women's Tennis Association rankings released.
Anastasija Sevastova rallied to eliminate the five-time Grand Slam champion Maria Sharapova at Arthur Ashe Stadium.
Venus Williams was forced to draw on all of her vast experience before overcoming Czech youngster Katerina Siniakova.
Muguruza stunned sentimental favourite Venus Williams 7-5, 6-0 on Saturday to clinch her second Grand Slam crown.
Garbine Muguruza said it felt "incredible" to become Wimbledon champion even if it did mean defeating childhood idol Venus Williams.
Garbine Muguruza stormed to her first Wimbledon title and shattered Venus Williams' history bid with a majestic 7-5, 6-0 victory.
Konta fought back to beat Halep 6-7 (2/7), 7-6 (7/5), 6-4 and now faces five-time Wimbledon champion Venus.