On Wednesday the New York mayor's office wrote the UNGA laying out the guidelines, including that delegates must show proof of vaccination to enter the debate hall.
Many of those protesting dressed all in white, as instructed by political groups that organised the demonstrations in at least 19 states.
Bolsonaro -- who has been squaring off publicly with the high court for weeks since it opened investigations against him and those around him -- took a menacing tone at the rally.
Bolsonaro rallied demonstrations on Tuesday to coincide with Independence Day and show strength as his approval ratings slide and he feuds with the Supreme Court.
Massive participation in rallies scheduled across the country would reinforce the right-wing leader's push to prove he is a strong candidate for reelection.
Bolsonaro supporters had reached the opposite end of the mall, where police stood guard behind metal barricades outside the Congress and the Supreme Court.
Without presenting any evidence, Bolsonaro has insisted Brazil’s electronic voting system is prone to fraud, and that printouts would allow for auditing results.
Bolsonaro has alleged that Brazil's electronic voting system isn't reliable and makes it impossible to audit results.
People in Brazil cast their ballots electronically at voting stations, and Bolsonaro has for years been arguing for a paper printout to be made of each vote cast.
In recent weeks, Bolsonaro has appeared to struggle with speaking on various occasions and said that he suffers from recurring hiccups.
Brazilian President Jair Bolsonaro has appeared to struggle with speaking on various occasions in recent weeks and said that he suffers from recurring hiccups.
Bolsonaro has said on various occasions that he suffers from recurring hiccups that can go uninterrupted for days.
Bolsonaro has denied any wrongdoing or knowledge of corruption, and told reporters on June 28 he can't know what transpires within his ministries.
Sao Paulo's state government press office said a fine - equivalent to about USD 110 - would be imposed for violation of a rule that has required masks in public places since May 2020.
His appointment in October 2019 came as Bolsonaro was projecting ideological alignment with the US and then-President Donald Trump.