Senior advocate S Shyamsundar said the SIT is duty bound to question IT officials since the incident took place when IT officials were present in the building.
In a letter dated Sunday, the Chief Minister said the incident showed grave negligence, as it was alarming that a person at an I-T search could access a loaded gun.
The last rites of Roy, who was found dead at his office on January 30 with bullet injuries, were performed on Sunday in the presence of family members, friends and admirers.