Rahul Dravid ensured game time for almost everyone in the squad as Nitish Rana, Rahul Chahar, Chetan Sakariya, Kishnappa Gowtham and Sanju Samson made their debuts.
Ishan Kishan is making his ODI debut while Samson's injury is being monitored by the medical team.
Captaincy was great learning experience for Sanju Samson, he was really growing into the role: Jos Buttler
Sanju Samson, who had finished as the side's highest run-getter in the 2020 season, had replaced Steve Smith as skipper of Rajasthan Royals in January.
The Royals chased down a modest 134-run target with ease for their second win of the season after their pacers restricted the rivals to 133 for nine.
While Jos Buttler's direct hit ended Gill's knock with KKR scoring 25 runs in the powerplay, KKR kept losing wickets at regular intervals and got reduced to 61/4 at the halfway mark.
IPL 2021: Rajasthan Royals captain Sanju Samson needs to lead from the front with bat, says Gavaskar
Samson scored a blistering 119 in the first match of the IPL season but has since managed scores of 4, 1 and 21.
RCB batsmen Padikkal and Kohli bludgeoned the Royals bowling attack and Samson admitted that this kind of defeat has put their morals down.
Samson scored a blistering ton in the first match which Royals lost narrowly to Punjab Kings but failed to come good against Delhi Capitals and against Chennai Super Kings on Monday night.
Both sides made two changes from their previous match playing eleven.
In his debut game, Chetan Sakariya returned with the impressive figures of 3-31 in his four overs even when Punjab Kings posted 221.
Having started off with ton, it will be interesting to see if RR skipper can keep up good work
Sanju Samson declined a single to Chris Morris off the second-last ball of the innings, while chasing a mammoth target of 221.
Punjab Kings managed to hold on but Sanju, despite added responsibility of being the captain, proved that he can be a match winner for his side.
Samson's fighting hundred went in vain as Arshdeep Singh held his nerves in the final over to help Punjab Kings register a thrilling win over Rajasthan Royals here at the Wankhede on Monday.
Samson almost took Royals home with a breathtaking 119 off 63 balls but was caught off the game's final ball with left-arm pacer Arshdeep defending 13 runs in the last over on Monday night.